Metabolic Phenotyping Facility


The facility & Equipment

The equipment for the service is located in either Advanced Technology Lab, Agricultural Biotechnology Lab, or R.E. Jones building. Below is a list of equipment, location and usage.
 
  • CLAMS (ABL animal facility 149): Metabolic cages (Oxymax CLAMS) are used in awake mice to measure energy expenditure, physical activity, indirect calorimetry, and food/water intake. The experiment non-invasively measures O2 consumption and CO2 production in individual mice using metabolic chambers and calculates the respiratory exchange ratio to reflect energy expenditure. Normally, mice are put in a CLAMS metabolic cage for 3 days. 
  • EchoMRI-100 (ABL animal facility 149): This equipment is a body composition analyzer for small animals (body weight <100 g) that can measure whole body fat, lean, free water, and total water masses in live animals.
  • Seahorse XFe24 analyzer (ATL 220): This analyzer determines rates of cellular energy metabolism, e.g., glycolysis and mitochondrial respiration.
  • Luminex MAGPIX for cytokine array (ATL 203): This equipment is to measure cytokines, chemokines, and hormones in plasma/serum samples.
  • Cobas clinical chemistry analyzer (Jones 117): This analyzer measures common clinical chemistry in human and animal blood samples.

Service Rates
 
  • CLAMS
  • $1,400/run + per diem for internal users
  • 8-10 mice max/run
  • Users should arrange mouse transportation to ABL animal facility for this service
  • EchoMRI-100
  • $300/run
  • 10 mice max/run
  • Users should arrange mouse transportation to ABL animal facility for this service
  • Seahorse XFe24 analyzer
  • $200/plate + cost for a cartridge for internal-unassisted users
  • $500/plate + cost for a cartridge for internal-assisted users  
  • The first time user should use “assisted service” for training until he/she can run the assay his/her own.
  • Luminex MAGPIX
  • $140/plate + cost for a kit/panel for internal-unassisted users
  • $200/plate + cost for a kit/panel for internal-assisted users 
  • Panel examples: human apolipoprotein panel, human cytokine panel, mouse cytokine panel
  • The first time user should use “assisted service” for training until he/she can run the assay his/her own.
  • Cobas clinical chemistry analyzer
  • 10 samples max/run
  • $350/run + cost for reagents/kit
  • Analysis for total cholesterol, LDL cholesterol, HDL cholesterol, triglycerides and glucose, C reactive protein, ALT and AST each run

External Rates

For external users, rates will ~35% higher than those for the UConn internal users to cover the university’s overhead rate.

For questions, please contact Dr. Young-Ki Park

 

Contact us

Tel: 860-486-3633


Email: nusc@uconn.edu


Roy E. Jones Building,
27 Manter Rd., Unit 4017
Storrs, CT 06269


8:00am – 4:00pm Monday through Friday

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